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Author Rodio, Maria-Elena ♦ Delgado, Sonia ♦ De Stradis, Angelo ♦ Gómez, María-Dolores ♦ Flores, Ricardo ♦ Di Serio, Francesco
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Viruses ♦ Organisms ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Microbiological Phenomena ♦ Plant Physiological Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Chloroplasts ♦ Virology ♦ Nucleic Acid Conformation ♦ Prunus ♦ Growth & Development ♦ Rna, Viral ♦ Chemistry ♦ Viroids ♦ Base Sequence ♦ Genetics ♦ Ultrastructure ♦ Gene Expression Regulation, Plant ♦ Meristem ♦ Cytology ♦ Models, Biological ♦ Molecular Sequence Data ♦ Mutation ♦ Phenotype ♦ Plant Diseases ♦ Plant Leaves ♦ Plant Proteins ♦ Metabolism ♦ Plant Viruses ♦ Physiology ♦ Protein Biosynthesis ♦ Rna, Messenger ♦ Rna, Ribosomal ♦ Seedling ♦ Solubility ♦ Transcription, Genetic ♦ Virus Replication ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Peach latent mosaic viroid (PLMVd) is a chloroplast-replicating RNA that propagates in its natural host, peach (Prunus persica), as a complex mixture of variants, some of which are endowed with specific structural and pathogenic properties. This is the case of variant PC-C40, with an insertion of 12 to 13 nucleotides that folds into a hairpin capped by a U-rich loop, which is responsible for an albino-variegated phenotype known as peach calico (PC). We have applied a combination of ultrastructural, biochemical, and molecular approaches to dissect the pathogenic effects of PC-C40. Albino sectors of leaves infected with variant PC-C40 presented palisade cells that did not completely differentiate into a columnar layer and altered plastids with irregular shape and size and with rudimentary thylakoids, resembling proplastids. Furthermore, impaired processing and accumulation of plastid rRNAs and, consequently, of the plastid translation machinery was observed in the albino sectors of leaves infected with variant PC-C40 but not in the adjacent green areas or in leaves infected by mosaic-inducing or latent variants (including PC-C40Delta, in which the 12- to 13-nucleotide insertion was deleted). Protein gel blot and RT-PCR analyses showed that the altered plastids support the import of nucleus-encoded proteins, including a chloroplast RNA polymerase, the transcripts of which were detected. RNA gel blot and in situ hybridizations revealed that PLMVd replicates in the albino leaf sectors and that it can invade the shoot apical meristem and induce alterations in proplastids, bypassing the RNA surveillance system that restricts the entry of a nucleus-replicating viroid and most RNA viruses. Therefore, a non-protein-coding RNA with a specific structural motif can interfere with an early step of the chloroplast developmental program, leading ultimately to an albino-variegated phenotype resembling that of certain variegated mutants in which plastid rRNA maturation is also impaired. Our results highlight the potential of viroids for further dissection of RNA trafficking and pathogenesis in plants.
Description Country affiliation: Italy
Author Affiliation: Rodio ME ( Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Istituto di Virologia Vegetale del Consiglio Nazionale delle Ricerche, Sezione di Bari, 70126 Bari, Italy.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-11-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 19
Issue Number 11


Source: WHO-Global Index Medicus