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Author Choudhury, Soumen ♦ Garg, Satish Kumar ♦ Singh, Thakur Uttam ♦ Mishra, Santosh Kumar
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Animal husbandry ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Musculoskeletal System ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Heterocyclic Compounds ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Animal science ♦ Discipline Reproductive Medicine ♦ Discipline Veterinary Medicine ♦ Discipline Zoology ♦ Large-conductance Calcium-activated Potassium Channels ♦ Physiology ♦ Myometrium ♦ Animals ♦ Benzimidazoles ♦ Pharmacology ♦ Blotting, Western ♦ Veterinary ♦ Buffaloes ♦ Metabolism ♦ Dose-response Relationship, Drug ♦ Female ♦ Analysis ♦ Antagonists & Inhibitors ♦ Chemistry ♦ Drug Effects ♦ Peptides ♦ Reverse Transcriptase Polymerase Chain Reaction ♦ Tetraethylammonium ♦ Uterine Contraction ♦ Journal Article
Abstract Large conductance potassium channels (BK(Ca) channels) play a central role in maintaining myometrial tone, thus activation of these channels proved to have therapeutic potential in preterm labor. Present study aims to unravel the presence of BK(Ca) (maxi-K) channels in buffalo myometrium. Tension experiments, mRNA and protein expression studies were done to characterize BK(Ca) channels in buffalo myometrium. Isolated myometrial preparations exhibited rhythmic spontaneity with regular pattern of amplitude and frequency. Selective blockers of BK(Ca) channels iberiotoxin (IbTx; 100nM) and tetraethylammonium (TEA; 1mM) produced excitatory effects as evidenced by increase in amplitude and frequency of myogenic activity. 1,3-Dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimi-dazol-2-one (NS-1619; 10(-7)-10(-4)M), a BK(Ca) channel opener, produced concentration-dependent relaxation of myometrium with pD(2) of 5.02±0.19 and R(max) of 31.35±3.5% (n=5). TEA significantly antagonized NS-1619-induced relaxation (pD(2) of 4.72±0.12 and R(max) of 22.72±1.78%; n=5). IbTx also significantly shifted the dose response curve of NS-1619 towards right (pD(2) of 3.98±0.16; n=4) without significant change in the per cent maximal response. Further, RT-PCR study detected mRNA encoding BK(Ca) -subunit and Western blot analysis detected its protein expression in myometrium. Based on the results of the present investigation, it is suggested that BK(Ca) channels are present in the buffalo myometrium and are open in the resting state. Thus, their activation by potassium channel opener/ß(2)-adrenoceptor agonist (tocolytic drug) may lead to uterine relaxation in preterm labor.
Description Country affiliation: India
Author Affiliation: Choudhury S ( Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, Pandit Deen Dayal Upadhyaya Veterinary Sciences University & Cattle Research Institute, Mathura, India.)
ISSN 03784320
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-07-01
Publisher Place Netherlands
e-ISSN 18732232
Journal Animal Reproduction Science
Volume Number 126
Issue Number 3-4

Source: WHO-Global Index Medicus