|Author||Choudhury, Soumen ♦ Garg, Satish Kumar ♦ Singh, Thakur Uttam ♦ Mishra, Santosh Kumar|
|Source||World Health Organization (WHO)-Global Index Medicus|
|File Format||HTM / HTML|
|Subject Domain (in DDC)||Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Animal husbandry ♦ Manufacture for specific uses ♦ Precision instruments & other devices|
|Subject Domain (in MeSH)||Musculoskeletal System ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Heterocyclic Compounds ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences|
|Subject Keyword||Discipline Animal science ♦ Discipline Reproductive Medicine ♦ Discipline Veterinary Medicine ♦ Discipline Zoology ♦ Large-conductance Calcium-activated Potassium Channels ♦ Physiology ♦ Myometrium ♦ Animals ♦ Benzimidazoles ♦ Pharmacology ♦ Blotting, Western ♦ Veterinary ♦ Buffaloes ♦ Metabolism ♦ Dose-response Relationship, Drug ♦ Female ♦ Analysis ♦ Antagonists & Inhibitors ♦ Chemistry ♦ Drug Effects ♦ Peptides ♦ Reverse Transcriptase Polymerase Chain Reaction ♦ Tetraethylammonium ♦ Uterine Contraction ♦ Journal Article|
|Abstract||Large conductance potassium channels (BK(Ca) channels) play a central role in maintaining myometrial tone, thus activation of these channels proved to have therapeutic potential in preterm labor. Present study aims to unravel the presence of BK(Ca) (maxi-K) channels in buffalo myometrium. Tension experiments, mRNA and protein expression studies were done to characterize BK(Ca) channels in buffalo myometrium. Isolated myometrial preparations exhibited rhythmic spontaneity with regular pattern of amplitude and frequency. Selective blockers of BK(Ca) channels iberiotoxin (IbTx; 100nM) and tetraethylammonium (TEA; 1mM) produced excitatory effects as evidenced by increase in amplitude and frequency of myogenic activity. 1,3-Dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimi-dazol-2-one (NS-1619; 10(-7)-10(-4)M), a BK(Ca) channel opener, produced concentration-dependent relaxation of myometrium with pD(2) of 5.02±0.19 and R(max) of 31.35±3.5% (n=5). TEA significantly antagonized NS-1619-induced relaxation (pD(2) of 4.72±0.12 and R(max) of 22.72±1.78%; n=5). IbTx also significantly shifted the dose response curve of NS-1619 towards right (pD(2) of 3.98±0.16; n=4) without significant change in the per cent maximal response. Further, RT-PCR study detected mRNA encoding BK(Ca) -subunit and Western blot analysis detected its protein expression in myometrium. Based on the results of the present investigation, it is suggested that BK(Ca) channels are present in the buffalo myometrium and are open in the resting state. Thus, their activation by potassium channel opener/ß(2)-adrenoceptor agonist (tocolytic drug) may lead to uterine relaxation in preterm labor.|
|Description||Country affiliation: India
Author Affiliation: Choudhury S ( Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, Pandit Deen Dayal Upadhyaya Veterinary Sciences University & Cattle Research Institute, Mathura, India.)
|Educational Role||Student ♦ Teacher|
|Age Range||above 22 year|
|Educational Use||Reading ♦ Research ♦ Self Learning|
|Education Level||UG and PG|
|Learning Resource Type||Article|
|Journal||Animal Reproduction Science|
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