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Author Hayashi, Yuki ♦ Kato, Kazashi ♦ Kimura, Keiji
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ACTINOMYCIN ♦ ALBUMINS ♦ CATTLE ♦ CHROMOSOMES ♦ MITOSIS ♦ PHOSPHATES ♦ RIBOSOMAL RNA ♦ URIDINE
Abstract The perichromosomal layer (PCL) is a structure that surrounds mitotic chromosomes, found in both animal and plant cells. It comprises various proteins and RNAs, mainly derived from the nucleolus. Several functions for the PCL have been suggested; however, the mechanism of PCL organization during mitosis remains unclear. The localization of several nucleolar proteins to the PCL is reportedly dependent on pre-ribosomal RNAs and the marker of proliferation, Ki67, which is a major PCL-localized protein. Here we demonstrate that, although the removal of pre-ribosomal RNAs from the PCL causes PCL delocalization of several nucleolar proteins, it does not affect the localization of Ki67. Conversely, Ki67 depletion results in the dissociation of both pre-ribosomal RNAs and nucleolar proteins from the PCL, which indicates that Ki67 is required for the PCL accumulation of pre-ribosomal RNAs, to which several nucleolar proteins are associated. Given these findings, we propose a model for PCL organization that comprises three essential layers: the scaffolding protein Ki67, pre-ribosomal RNAs for linkage, and outer nucleolar proteins. - Highlights: • Pre-rRNAs are required for PCL association of several nucleolar proteins. • Pre-rRNAs on the PCL are dispensable for PCL localization of Ki67. • Ki67 is essential for association of pre-rRNAs on the PCL. • PCL consists of inner Ki67, intermediate pre-rRNAs, and outer nucleolar proteins.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-11-18
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 493
Issue Number 2


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