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Author Karagounis, Ilias V. ♦ Koukourakis, Michael I. ♦ Abatzoglou, Ioannis M.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ RADIATION PROTECTION AND DOSIMETRY ♦ ABDOMEN ♦ ANESTHESIA ♦ CHEST ♦ COMPUTERIZED TOMOGRAPHY ♦ DOSIMETRY ♦ HEAD ♦ IMAGE PROCESSING ♦ IN VIVO ♦ MICE ♦ ORGANS ♦ PARTIAL BODY IRRADIATION ♦ PBI ♦ PMMA ♦ RADIATION DOSES ♦ RADIOBIOLOGY ♦ RADIOTHERAPY ♦ WHOLE-BODY IRRADIATION
Abstract Purpose: In vivo radiobiology experiments involving partial body irradiation (PBI) of mice are of major importance because they allow for the evaluation of individual organ tolerance; overcoming current limitations of experiments using lower dose, whole body irradiation. In the current study, the authors characterize and validate an effective and efficient apparatus for multiple animal PBI, directed to the head, thorax, or abdomen of mice. Methods: The apparatus is made of polymethylmethacrylate and consists of a rectangular parallelepiped prism (40 cm × 16 cm × 8 cm), in which five holes were drilled to accomodate standard 60 ml syringes, each housing an unanesthetized, fully immobilized mouse. Following CT-scanning and radiotherapy treatment planning, radiation fields were designed to irradiate the head, thorax, or abdomen of the animal. Thermoluminescent dosimeters (TLDs) were used to confirm the treatment planning dosimetry for primary beam and scattered radiation. Results: Mice are efficiently placed into 60 ml syringes and immobilized, without the use of anesthetics. Although partial rotational movement around the longitudinal axis and a minor 2 mm forward/backward movement are permitted, this does not compromise the irradiation of the chosen body area. TLDs confirmed the dose values predicted by the treatment planning dosimetry, both for primary beam and scattered radiation. Conclusions: The customized PMMA apparatus described and validated is cost-effective, convenient to use, and efficient in performing PBI without the use of anesthesia. The developed apparatus permits the isolated irradiation of the mouse head, thorax, and abdomen. Importantly, the apparatus allows the delivery of PBI to five mice, simultaneously, representing an efficient way to effectively expose a large number of animals to PBI through multiple daily fractions, simulating clinical radiotherapy treatment schedules.
ISSN 00942405
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-15
Publisher Place United States
Journal Medical Physics
Volume Number 43
Issue Number 5


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