Thumbnail
Access Restriction
Open

Author Lu, Ting ♦ Bao, Zhen ♦ Wang, Yunfeng ♦ Yang, Lixiang ♦ Lu, Bing ♦ Yan, Ke ♦ Wang, Shaozhen ♦ Wei, He ♦ Zhang, Zhe ♦ Cui, Gang
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIOLOGY AND NUCLEAR MEDICINE ♦ APPLIED LIFE SCIENCES ♦ GLIOMAS ♦ HEAD ♦ NUCLEI
Abstract Karyopherinβ1 (KPNB1), one of the cytosolic factors involved in the selective protein transport across nucleus, docked at nuclear pore complex and transported through nuclear envelope in an ATP-dependent style, assisting proteins to be recognized as import substrates. It has been reported to be bound up with the origination and progress of lung cancer, cervical cancer, head and neck cancer and hepatocellular carcinoma. In current study, we demonstrated for the first time that the role of KPNB1 in human glioma. KPNB1 was over-expressed as the well-known trend of Ki-67(p < 0.01) and tightly closed to poor prognosis, as an independent prognostic factor. In vitro, up-regulation of KPNB1 was accompanied by certain rising levels of proliferation markers, employing U251 and U87MG cells as serum-starve models. Silencing KPNB1 in U251 and U87MG led to G1 phase arrested directly via flow cytometry analysis. In the nucleus of KPNB1-depletion cell models, the decreasing expression of KPNB1 and β-catenin was detected respectively, which indicated that KPNB1 functioned via β-catenin signal. Besides, the interaction between KPNB1 and β-catenin was proved clearly by immunoprecipitation. Taken together, it showed that KPNB1 might enhance human glioma proliferation via Wnt/β-Catenin Pathway. - Highlights: • KPNB1 accelerated the progress of human glioma via Wnt/β-catenin pathway. • The decreased β-catenin expression in nucleus of KPNB1-depletion cells. • We identified the interaction between β-catenin and KPNB1 for the first time. • The close positive relationship between KPNB1 expression and glioma proliferation. • Two kinds of glioma cell lines were applied to this study.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-09-23
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 478
Issue Number 3


Open content in new tab

   Open content in new tab