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Author Wu, Yuan-Yuan ♦ Zhang, Jing-Hua ♦ Gao, Jing-Hua ♦ Li, Yong-Sheng
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ADP ♦ ALOE ♦ ANTHRAQUINONES ♦ APOPTOSIS ♦ AUGER ELECTRON SPECTROSCOPY ♦ CARCINOMAS ♦ CELL PROLIFERATION ♦ CHEMOTHERAPY ♦ GLYCOLIC ACID ♦ IN VITRO ♦ IN VIVO ♦ LUNGS ♦ MICE ♦ NANOPARTICLES ♦ PHOSPHOTRANSFERASES ♦ POLYMERASES ♦ RIBOSE ♦ TUMOR CELLS
Abstract Human lung squamous cell carcinoma is a deadly cancer for which present therapeutic strategies are inadequate. And traditional chemotherapy results in severe systemic toxicity. Compounds from living organisms often exert a biological activity, triggering several targets, which may be useful for the improvement of novel pharmaceuticals. Aloe-emodin (AE), a well-known natural compound, is a primary component of anthraquinones in Aloe vera and exhibits anti-proliferative and apoptotic effects on various tumor cells. However, the translational and clinical use of AE has been limited owing to its rapid degradation and poor bioavailability. To improve its efficacy, a poly (lactic-co-glycolic acid) based AE nanoparticle formulation (NanoAE) was prepared. Our study indicated that compared to the free AE, nanoAE significantly suppressed cancer cell proliferation, induced cell cycle arrest and apoptosis, evidenced by high cleavage of Caspase-3, poly (ADP-ribose) polymerase (PARP), Caspase-8 and Caspase-9. NanoAE enhanced reactive oxygen species (ROS) production, along with Mitogen-activated protein kinases (MAPKs) activation and PI3K/AKT inactivation. Cell proliferation, apoptosis and MAPKs and PI3K/AKT were dependent on ROS production in nanoAE-treated groups. In vivo, nanoAE exhibited inhibitory effects on the tumor growth with little toxicity. Together, our results indicated that nanoAE might be an effective treatment for human lung squamous cell carcinoma. - Highlights: • Aloe-emodin nanoparticles (NanoAE) displayed significant anti-tumor effect in lung squamous carcinoma. • NanoAE inhibited cell proliferation and induced apoptosis in squamous carcinoma cells. • NanoAE up-regulated ROS levels in apoptosis and cell proliferation process. • NanoAE suppresses the growth of human squamous carcinoma mice in vivo.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-08-26
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 490
Issue Number 3


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