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Author Mallick, Sumana ♦ Pal, Bikas Chandra ♦ Kumar, Deepak ♦ Chatterjee, Nabanita ♦ Das, Subhadip ♦ Saha, Krishna D.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Taylor & Francis
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Neoplasms ♦ Diseases ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Metabolism ♦ Cell Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Chemistry ♦ Discipline Pharmacology ♦ Antineoplastic Agents, Phytogenic ♦ Pharmacology ♦ Corchorus ♦ Chemistry ♦ Glycosides ♦ Triterpenes ♦ Animals ♦ Isolation & Purification ♦ Apoptosis ♦ Drug Effects ♦ Caspase 9 ♦ Metabolism ♦ Humans ♦ Melanoma ♦ Pathology ♦ Physiopathology ♦ Membrane Potential, Mitochondrial ♦ Mice ♦ Mitochondria ♦ Molecular Structure ♦ Oleanolic Acid ♦ Analogs & Derivatives ♦ Saponins ♦ Skin Neoplasms ♦ Bcl-2-associated X Protein ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Corchorusin-D (COR-D), isolated from Corchorus acutangulus, was reported to induce apoptosis in leukemic cells. However, no studies concerning its activity on melanoma cells have been reported. We have evaluated its in vitro anti-cancer activity on melanoma cells (B16F10, SK-MEL-28, and A375). The results demonstrate that COR-D showed maximum inhibition of B16F10 cells in vitro. COR-D induced mitochondrial dysfunction and altered the Bax/Bcl-2 ratio with down regulation of pro-caspases 9 and activation of caspase 3 in B16F10 cells, triggering intrinsic pathway of apoptosis. Moreover, it inhibited the in vivo B16F10 tumor growth and increased the survival rate of mice. Greater number of Annexin V-FITC and propidium iodide (PI)-positive tumor cells signified that COR-D induced apoptosis in vivo also. The reduction in tumor growth is well correlated with decreased microvascular density of the tumor cells in treated mice. In conclusion, this study reveals that COR-D-induced mitochondrial dysfunction is responsible for the induction of apoptotic cell death.
Description Country affiliation: India
Author Affiliation: Mallick S ( a Cancer & Cell Biology Division, CSIR Indian Institute of Chemical Biology , 4, Raja S. C. Mullick Road, Jadavpur Kolkata 700032 , India.)
ISSN 10286020
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-11-01
Publisher Place Great Britain (UK)
e-ISSN 14772213
Journal Journal of Asian Natural Products Research
Volume Number 15
Issue Number 11

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Source: WHO-Global Index Medicus