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Author Tan, Zheng-Huai ♦ Yu, Ling-Hong ♦ Wei, Huai-Ling ♦ Liu, Geng-Tao
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Taylor & Francis
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases
Subject Domain (in MeSH) Respiratory System ♦ Anatomy ♦ Eukaryota ♦ Bacteria ♦ Organisms ♦ Respiratory Tract Diseases ♦ Diseases ♦ Organic Chemicals ♦ Heterocyclic Compounds ♦ Macromolecular Substances ♦ Enzymes and Coenzymes ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Biological Factors ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Chemistry ♦ Discipline Pharmacology ♦ Acute Lung Injury ♦ Chemically Induced ♦ Apigenin ♦ Pharmacology ♦ Glucuronates ♦ Lipopolysaccharides ♦ Nf-kappa B ♦ Drug Effects ♦ Tumor Necrosis Factor-alpha ♦ Metabolism ♦ Actins ♦ Blood ♦ Animals ♦ Endotoxins ♦ Escherichia Coli ♦ Chemistry ♦ Genes, Fos ♦ Lung ♦ Mice ♦ Molecular Structure ♦ Nitric Oxide Synthase Type Ii ♦ Rna, Messenger ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract This paper investigates the effect of natural scutellarin on acute lung injury (ALI) induced by Escherichia coli endotoxin lipopolysaccharide (LPS) in mice and its mechanism of action. Mouse ALI was induced by the injection of LPS (15 mg/kg) via the tail vein, and mice were intraperitoneally injected with 50 and 25 mg/kg of scutellarin before the LPS injection. The lung index, serum NO2(-)/NO3(-), and tumor necrosis factor-alpha (TNF-alpha) levels were determined using kits. The lung lesions were examined by light microscope. The mRNA levels of TNF-alpha, inducible nitric oxide synthase (iNOS), and FasL in pulmonary tissues were detected by RT-PCR. c-Fos, c-Jun, IkappaB, and iNOS proteins were detected by the western blotting method. Pretreatment with 25 and 50 mg/kg of scutellarin significantly reduced lung injury induced by LPS, which expressed in the decrease in lung morphological lesions, serum NO2(-)/NO3(-), TNF-alpha levels, lactate dehydrogenase release, and total protein in the lavage fluid of bronchoalveolar of the lung. The mRNA level of TNF-alpha, iNOS, the protein content of c-Fos, iNOS, and the activation of NF-kappaB in pulmonary tissues were all inhibited, while the lung glutathione level increased. In conclusion, scutellarin has protective action against LPS-induced lung damage in mice, and its underlying mechanism might be the inhibition of IkappaB alpha degradation and the expression of TNF-alpha mRNA.
Description Country affiliation: China
Author Affiliation: Tan ZH ( Department of Pharmacology, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.)
ISSN 10286020
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-03-01
Publisher Place Great Britain (UK)
e-ISSN 14772213
Journal Journal of Asian Natural Products Research
Volume Number 12
Issue Number 3

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Source: WHO-Global Index Medicus