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Author Ali, Mubarak ♦ Ahmed, Ishtiaq ♦ Ramirez, Patricio ♦ Nasir, Saima ♦ Niemeyer, Christof M. ♦ Mafe, Salvador ♦ Ensinger, Wolfgang
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley-VCH
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Technology ♦ Medicine & health
Abstract The label-free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2-picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc $(Zn^{2+})$ ion is achieved by exposing the modified pore to a solution of zinc chloride to form $bis(Zn^{2+}–DPA)$ complexes. The chemical functionalization is demonstrated by recording the changes in the observed current–voltage (I–V) curves before and after pore modification. The $bis(Zn^{2+}–DPA)$ complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson–Nernst–Planck equations.
Description Country affiliation: Germany
Author Affiliation: Ali M ( Department of Material- and Geo-Sciences, Materials Analysis, Technische Universität Darmstadt, D-64287, Darmstadt, Germany.); Ahmed I ( Materials Research Department, GSI Helmholtzzentrum für Schwerionenforschung, D-64291, Darmstadt, Germany.); Ramirez P ( Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), D-76344, Eggenstein-Leopoldshafen, Germany.); Nasir S ( Departamento de Física Aplicada, Universitat Politécnica de València, E-46022, Valencia, Spain.); Niemeyer CM ( Department of Material- and Geo-Sciences, Materials Analysis, Technische Universität Darmstadt, D-64287, Darmstadt, Germany.); Mafe S ( Materials Research Department, GSI Helmholtzzentrum für Schwerionenforschung, D-64291, Darmstadt, Germany.); Ensinger W ( Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), D-76344, Eggenstein-Leopoldshafen, Germany.)
ISSN 16136810
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2016-04-01
Publisher Place Germany
e-ISSN 16136829
Journal Small
Volume Number 12
Issue Number 15


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Source: WHO-Global Index Medicus