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Author Crouzier, Thomas ♦ Ren, Kefeng ♦ Nicolas, Claire ♦ Roy, Christian ♦ Picart, Catherine
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley-VCH
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Agriculture & related technologies
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Amino Acids, Peptides, and Proteins ♦ Biomedical and Dental Materials ♦ Pharmaceutical Preparations ♦ Chemicals and Drugs ♦ Physical Phenomena ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Biological Sciences ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Discipline Nanotechnology ♦ Biomimetic Materials ♦ Chemistry ♦ Bone Morphogenetic Proteins ♦ Pharmacology ♦ Drug Carriers ♦ Myoblasts ♦ Cytology ♦ Drug Effects ♦ Osteoblasts ♦ Recombinant Proteins ♦ Transforming Growth Factor Beta ♦ Animals ♦ Bone Morphogenetic Protein 2 ♦ Cell Differentiation ♦ Cell Line ♦ Diffusion ♦ Membranes, Artificial ♦ Mice ♦ Osteogenesis ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Efficient delivery of growth or survival factors to cells is one of the most important long-term challenges of current cell-based tissue engineering strategies. The extracellular matrix acts as a reservoir for a number of growth factors through interactions with its components. In the matrix, growth factors are protected against circulating proteases and locally concentrated. Thus, the localized and long-lasting delivery of a matrix-bound recombinant human bone morphogenetic protein 2 (rhBMP-2) from a biomaterial surface would mimic in vivo conditions and increase BMP-2 efficiency by limiting its degradation. Herein, it is shown that crosslinked poly(L-lysine)/hyaluronan (HA) layer-by-layer films can serve as a reservoir for rhBMP-2 delivery to myoblasts and induce their differentiation into osteoblasts in a dose-dependent manner. The amount of rhBMP-2 loaded in the films is controlled by varying the deposition conditions and the film thickness. Its local concentration in the film is increased up to approximately 500-fold when compared to its initial solution concentration. Its adsorption on the films, as well as its diffusion within the films, is evidenced by microfluorimetry and confocal microscopy observations. A direct interaction of rhBMP-2 with HA is demonstrated by size-exclusion chromatography, which could be at the origin of the rhBMP-2 'trapping' in the film and of its low release from the films. The bioactivity of rhBMP-2-loaded films is due neither to film degradation nor to rhBMP-2 release. The rhBMP-2-containing films are extremely resistant and could sustain three successive culture sequences while remaining bioactive, thus confirming the important and protective effect of rhBMP-2 immobilization. These films may find applications in the local delivery of immobilized growth factors for tissue-engineered constructs and for metallic biomaterial surfaces, as they can be deposited on a wide range of substrates with different shapes, sizes, and composition.
Description Country affiliation: France
Author Affiliation: Crouzier T ( UMR 5235 Dynamique des Interactions Membranaires Normales et Pathologiques CNRS, Université Montpellier II et I cc 107, Montpellier, France.)
ISSN 16136810
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-03-01
Publisher Place Germany
e-ISSN 16136829
Journal Small
Volume Number 5
Issue Number 5


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Source: WHO-Global Index Medicus