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Author Elbakry, Asmaa ♦ Wurster, Eva-Christina ♦ Zaky, Alaa ♦ Liebl, Renate ♦ Schindler, Edith ♦ Bauer-Kreisel, Petra ♦ Blunk, Torsten ♦ Rachel, Reinhard ♦ Goepferich, Achim ♦ Breunig, Miriam
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley-VCH
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Diagnosis ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Metabolism ♦ Biological Sciences ♦ Natural Science Disciplines ♦ Physical Sciences ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Discipline Nanotechnology ♦ Dna ♦ Administration & Dosage ♦ Gold ♦ Metal Nanoparticles ♦ Animals ♦ Base Sequence ♦ Biological Transport, Active ♦ Cho Cells ♦ Coated Materials, Biocompatible ♦ Cricetinae ♦ Cricetulus ♦ Genetics ♦ Drug Delivery Systems ♦ Hela Cells ♦ Humans ♦ Mcf-7 Cells ♦ Chemistry ♦ Ultrastructure ♦ Microscopy, Electron, Transmission ♦ Nanotechnology ♦ Particle Size ♦ Polyethyleneimine ♦ Rna, Small Interfering ♦ Surface Plasmon Resonance ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Because nanoparticles are finding uses in myriad biomedical applications, including the delivery of nucleic acids, a detailed knowledge of their interaction with the biological system is of utmost importance. Here the size-dependent uptake of gold nanoparticles (AuNPs) (20, 30, 50 and 80 nm), coated with a layer-by-layer approach with nucleic acid and poly(ethylene imine) (PEI), into a variety of mammalian cell lines is studied. In contrast to other studies, the optimal particle diameter for cellular uptake is determined but also the number of therapeutic cargo molecules per cell. It is found that 20 nm AuNPs, with diameters of about 32 nm after the coating process and about 88 nm including the protein corona after incubation in cell culture medium, yield the highest number of nanoparticles and therapeutic DNA molecules per cell. Interestingly, PEI, which is known for its toxicity, can be applied at significantly higher concentrations than its IC(50) value, most likely because it is tightly bound to the AuNP surface and/or covered by a protein corona. These results are important for the future design of nanomaterials for the delivery of nucleic acids in two ways. They demonstrate that changes in the nanoparticle size can lead to significant differences in the number of therapeutic molecules delivered per cell, and they reveal that the toxicity of polyelectrolytes can be modulated by an appropriate binding to the nanoparticle surface.
Description Country affiliation: Germany
Author Affiliation: Elbakry A ( Department of Pharmaceutical Technology, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany.)
ISSN 16136810
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-12-21
Publisher Place Germany
e-ISSN 16136829
Journal Small
Volume Number 8
Issue Number 24


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Source: WHO-Global Index Medicus