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Author Staedtke, V. ♦ Brähler, M. ♦ Müller, A. ♦ Georgieva, R. ♦ Bauer, S. ♦ Sternberg, N. ♦ Voigt, A. ♦ Lemke, A. ♦ Keck, C. ♦ Möschwitzer, J. ♦ Bäumler, H.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley-VCH
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Complex Mixtures ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Discipline Nanotechnology ♦ Amphotericin B ♦ Administration & Dosage ♦ Chemistry ♦ Candida Albicans ♦ Drug Effects ♦ Erythrocytes ♦ Cytology ♦ Microbiology ♦ Macrophages ♦ Metabolism ♦ Nanostructures ♦ Antifungal Agents ♦ Cells, Cultured ♦ Drug Compounding ♦ Humans ♦ Materials Testing ♦ Suspensions ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract The efficacy of antifungal treatment has been diminished by the biodistribution limitations of amphotericin B (AmB) due to its pharmacological profile, as well as the severe side effects it causes. A cellular drug-delivery system, which incorporates human erythrocytes (RBCs) loaded with an AmB nanosuspension (AmB-NS), is developed in order to improve antifungal treatment. AmB-NS encapsulation in RBCs is achieved by using hypotonic hemolysis, leading to intracellular AmB amounts of 3.81 +/- 0.47 pg RBC(-1) and an entrapment efficacy of 15-18%. Upon phagocytosis of AmB-NS-RBCs, leukocytes show a slow AmB release over ten days, and no alteration in cell viability. This results in an immediate, permanent inhibition of intra- and extracellular fungal activity. AmB-NS-RBC-leukocyte-mediated delivery of AmB is efficient in amounts 1000 times lower than the toxic dose. This drug-delivery method is effective for the transport of water-insoluble substances, such as AmB, and this warrants consideration for further testing.
Description Country affiliation: Germany
Author Affiliation: Staedtke V ( Center of Tumor Medicine, Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin, Berlin Charitéplatz 1, 10117 Berlin, Germany.)
ISSN 16136810
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-01-01
Publisher Place Germany
e-ISSN 16136829
Journal Small
Volume Number 6
Issue Number 1

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Source: WHO-Global Index Medicus