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Author Hu, Shuhuan ♦ Liu, Guangyu ♦ Chen, Weiqiang ♦ Li, Xiang ♦ Lu, Wei ♦ Lam, Raymond H. W. ♦ Fu, Jianping
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley-VCH
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Technology ♦ Medicine & health
Abstract Deep phenotyping of single cancer cells is of critical importance in the era of precision medicine to advance understanding of relationships between gene mutation and cell phenotype and to elucidate the biological nature of tumor heterogeneity. Existing microfluidic single-cell phenotyping tools, however, are limited to phenotypic measurements of 1-2 selected morphological and physiological features of single cells. Herein a microfluidic elasticity microcytometer is reported for multiparametric biomechanical and biochemical phenotypic profiling of free-floating, live single cancer cells for quantitative, simultaneous characterizations of cell size, cell deformability/stiffness, and surface receptors. The elasticity microcytometer is implemented for measurements and comparisons of four human cell lines with distinct metastatic potentials and derived from different human tissues. An analytical model is developed from first principles for the first time to convert cell deformation and adhesion information of single cancer cells encapsulated inside the elasticity microcytometer to cell deformability/stiffness and surface protein expression. Together, the elasticity microcytometer holds great promise for comprehensive molecular, cellular, and biomechanical phenotypic profiling of live cancer cells at the single cell level, critical for studying intratumor cellular and molecular heterogeneity using low-abundance, clinically relevant human cancer cells.
Description Country affiliation: Hong Kong
Author Affiliation: Hu S ( Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 852, Hong Kong.); Liu G ( Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.); Chen W ( Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.); Li X ( Department of Mechanical and Aerospace Engineering, New York University, NY, 11201, USA.); Lu W ( Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.); Lam RH ( Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.); Fu J ( Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 852, Hong Kong.)
ISSN 16136810
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2016-05-01
Publisher Place Germany
e-ISSN 16136829
Journal Small
Volume Number 12
Issue Number 17


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Source: WHO-Global Index Medicus