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Author Jin, Haojie ♦ Wang, Cun ♦ Gu, Dishui ♦ Zhang, Yurong ♦ Fan, Shaohua ♦ Xing, Shunpeng ♦ Wang, Hui ♦ Ruan, Haoyu ♦ Yang, Cheng ♦ Lv, Yuanyuan ♦ Feng, Hugang ♦ Yao, Ming
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CELL CYCLE ♦ HEPATECTOMY ♦ HYPERTROPHY ♦ KNOCK-OUT REACTIONS ♦ LIVER ♦ LIVER CELLS ♦ MICE ♦ PHOSPHORYLATION ♦ PROTEINS ♦ REGENERATION
Abstract The capacity of liver regeneration is critical for patients with liver diseases. However, cellular and molecular mechanisms of liver regeneration are still incompletely defined. Here, we assessed roles of LASS2 in liver regeneration following partial hepatectomy (PHx) in mice. Our results showed that protein level of LASS2 remarkably increased during liver regeneration after PHx in wildtype (WT) mice. Comparing to WT mice, liver regeneration index after PHx was significantly decreased from day 1 to day 5 in liver-specific LASS2 knockout (LASS2-LKO) mice. Interestingly, liver mass of LASS2-LKO mice could sufficiently recover at day 14 after PHx. Immunohistochemistry (IHC) and western blot analyses revealed that proliferation markers, such as PCNA and Ki67, were potently reduced during liver regeneration in LASS2-LKO mice. In addition, several cell cycle related molecules, such as cyclin A, CDK2 and p-Rb, were decreased in LASS2-LKO mice after PHx. Co-immunoprecipitation assay further revealed a decreased formation of CDK4/cyclin D1 complex after PHx in LASS2-LKO mice. However, phosphorylation of Akt was significantly activated from day 2 after PHx in LASS2-LKO mice when compared with that in WT mice, which may explain the recovery of liver mass at the late stage of liver regeneration in LASS2-LKO mice. Taken together, we conclude that LASS2 plays an important role in efficient liver regeneration in response to PHx. - Highlights: • LASS2 remarkably increased during liver regeneration after PHx. • Liver regeneration is delayed by LASS2 knockout in mice. • Proliferation and cell cycle entry of hepatocytes after PHx is impaired by LASS2 knockout in mice. • Knockout of LASS2 activated Akt signaling and increased cell size of hepatocytes during liver regeneration.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-11-25
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 493
Issue Number 3


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