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Author Wu, Jiangchun ♦ Wang, Ruobing ♦ Ye, Zhouheng ♦ Sun, Xuejun ♦ Chen, Zeli ♦ Xia, Fangzhou ♦ Sun, Qinglei ♦ Liu, Lin
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ DIABETES MELLITUS ♦ EOSIN ♦ EVANS BLUE ♦ GLUCOSE ♦ HEMATOXYLIN ♦ INFLAMMATION ♦ LEUKOCYTES ♦ LYMPHOKINES ♦ METHANE ♦ NECROSIS ♦ NEOPLASMS ♦ OXIDATION ♦ PERMEABILITY ♦ POLYMERASE CHAIN REACTION ♦ RATS ♦ STREPTOZOCIN ♦ TYROSINE ♦ VASCULAR DISEASES
Abstract As the commonest complication of diabetes mellitus (DM), diabetic retinopathy (DR) is a neuro-vascular disease with chronic inflammatory. Methane could exert potential therapeutic interest in inflammatory pathologies in previous studies. Our study aims to evaluate the protective effects of methane-rich saline on DR and investigate the potential role of related MicroRNA (miRNA) in diabetic rats. Streptozotocin-induced diabetic Sprague–Dawley rats were injected intraperitoneally with methane-rich or normal saline (5 ml/kg) daily for eight weeks. Morphology changes and blood-retinal barrier (BRB) permeability were assessed by hematoxylin eosin staining and Evans blue leakage. Retinal inflammatory cytokines levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL1-β) were evaluated by immunohistochemistry. Retinal protein expressions of glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) were determined by western blotting. Retinal miRNA expressions were examined by miRNA-specific microarray, verified by quantitative RT-PCR and predicted by GO enrichment and KEGG pathway analysis. There was no significant changes in blood glucose level and body weight of diabetic rats with methane-rich or normal saline treatment, but the decreased retinal thickness, retinal ganglial cell loss and BRB breakdown were all significantly suppressed by methane treatment. DM-induced retinal overexpressions of TNF-α, IL-1β, GFAP and VEGF were also significantly ameliorated. Moreover, the methane treatment significantly up-regulated retinal levels of miR-192-5p (related to apoptosis and tyrosine kinase signaling pathway) and miR-335 (related to proliferation, oxidative stress and leukocyte). Methane exerts protective effect on DR via anti-inflammation, which may be related to the regulatory mechanism of miRNAs. - Highlights: • Methane exerts protective effect on diabetic retinopathy via anti-inflammation. • Therapeutic effect of methane is related to the regulatory mechanism of miRNAs. • As a therapeutic gas, methane will be a bright future.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-10-16
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 466
Issue Number 2


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