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Author Tao, Ye ♦ Chen, Tao ♦ Liu, Bei ♦ Yang, Guo Qing ♦ Peng, Guanghua ♦ Zhang, Hua ♦ Huang, Yi Fei
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS ♦ APPLIED LIFE SCIENCES ♦ EFFICIENCY ♦ GAIN ♦ INFORMATION ♦ METHYL NITROSOUREA ♦ MICROSTRUCTURE ♦ NERVE CELLS ♦ PATHOLOGY ♦ RATS ♦ RECORDING SYSTEMS ♦ RETINA ♦ SIGNALS ♦ TIME DEPENDENCE ♦ TRANSMISSION ♦ VULNERABILITY
Abstract The neurotoxic effects of N-methyl-N-nitrosourea (MNU) on the inner retinal neurons and related visual signal circuits have not been described in any animal models or human, despite ample morphological evidences about the MNU induced photoreceptor (PR) degeneration. With the helping of MEA (multielectrode array) recording system, we gained the opportunity to systemically explore the neural activities and visual signal pathways of MNU administrated rats. Our MEA research identified remarkable alterations in the electrophysiological properties and firstly provided instructive information about the neurotoxicity of MNU that affects the signal transmission in the inner retina. Moreover, the spatial electrophysiological functions of retina were monitored and found that the focal PRs had different vulnerabilities to the MNU. The MNU-induced PR dysfunction exhibited a distinct spatial- and time-dependent progression. In contrast, the spiking activities of both central and peripheral RGCs altered synchronously in response to the MNU administration. Pharmacological tests suggested that gap junctions played a pivotal role in this homogeneous response of RGCs. SNR analysis of MNU treated retina suggested that the signaling efficiency and fidelity of inner retinal circuits have been ruined by this toxicant, although the microstructure of the inner retina seemed relatively consolidated. The present study provided an appropriate example of MEA investigations on the toxicant induced pathological models and the effects of the pharmacological compounds on neuron activities. The positional MEA information would enrich our knowledge about the pathology of MNU induced RP models, and eventually be instrumental for elucidating the underlying mechanism of human RP. - Highlights: • We systemically explored the neural activities and visual signal pathways of MNU administrated retinas. • The focal photoreceptors had different vulnerabilities to the MNU administration. • Pharmacological tests suggested that gap junctions played a pivotal role in this homogeneous response of RGCs. • The present study provided an appropriate example of MEA investigations on the toxicant induced pathological models. • The MEA information would enrich our knowledge about the pathology of MNU induced RP models.
ISSN 0041008X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-07-01
Publisher Place United States
Journal Toxicology and Applied Pharmacology
Volume Number 286
Issue Number 1


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