Thumbnail
Access Restriction
Open

Author Saifer, Abraham ♦ Steigman, Joseph
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword CHEMISTRY ♦ ACIDS ♦ ALBUMINS ♦ BINDING ENERGY ♦ BLOOD SERUM ♦ DIALYSIS ♦ DONNAN THEORY ♦ HYDROGEN ♦ IODIDES ♦ IODINE 131 ♦ IONS ♦ MEASURED VALUES ♦ MEMBRANES ♦ POTASSIUM IODIDES ♦ PROTEINS ♦ REACTION KINETICS ♦ SODIUM 24 ♦ SODIUM IODIDES ♦ SOLUTIONS ♦ TRACER TECHNIQUES ♦ USES
Abstract Binding of iodide ion from solutions of sodium or potassium iodide to deionized bovine serum albumin (BSA) and to bovine serum mercaptalbumin (BSM) was studied by equilibrium dialysis using radioactive I¹³¹ as a tracer. The Donnan effect was evaluated by introducing another radioactive ion, e.g., Nail, into the system which does not bind to these proteins. In acid solutions of BSA unbound hydrogen ions measured by pH showed the same distribution across the membrane as did the Na²⁴ions. In addition, at pH 12, where iodide ion should not bind, the I¹³¹ ratio across the membrane was equal to the reciprocal of the Na²⁴ ratio. Finally at isoionic pH, the binding constants for iodide ion to BSM determined by this method were shown to be in close agreement with those obtained by other workers by means of e.m.f. measurements. (auth)
ISSN 00223654
Educational Use Research
Learning Resource Type Article
Publisher Date 1961-01-01
Publisher Department Polytechnic Inst., Brooklyn
Journal Journal of Physical Chemistry
Volume Number 65
Issue Number 1
Organization Polytechnic Inst., Brooklyn


Open content in new tab

   Open content in new tab