|Author||Saifer, Abraham ♦ Steigman, Joseph|
|Source||United States Department of Energy Office of Scientific and Technical Information|
|Subject Keyword||CHEMISTRY ♦ ACIDS ♦ ALBUMINS ♦ BINDING ENERGY ♦ BLOOD SERUM ♦ DIALYSIS ♦ DONNAN THEORY ♦ HYDROGEN ♦ IODIDES ♦ IODINE 131 ♦ IONS ♦ MEASURED VALUES ♦ MEMBRANES ♦ POTASSIUM IODIDES ♦ PROTEINS ♦ REACTION KINETICS ♦ SODIUM 24 ♦ SODIUM IODIDES ♦ SOLUTIONS ♦ TRACER TECHNIQUES ♦ USES|
|Abstract||Binding of iodide ion from solutions of sodium or potassium iodide to deionized bovine serum albumin (BSA) and to bovine serum mercaptalbumin (BSM) was studied by equilibrium dialysis using radioactive I¹³¹ as a tracer. The Donnan effect was evaluated by introducing another radioactive ion, e.g., Nail, into the system which does not bind to these proteins. In acid solutions of BSA unbound hydrogen ions measured by pH showed the same distribution across the membrane as did the Na²⁴ions. In addition, at pH 12, where iodide ion should not bind, the I¹³¹ ratio across the membrane was equal to the reciprocal of the Na²⁴ ratio. Finally at isoionic pH, the binding constants for iodide ion to BSM determined by this method were shown to be in close agreement with those obtained by other workers by means of e.m.f. measurements. (auth)|
|Learning Resource Type||Article|
|Publisher Department||Polytechnic Inst., Brooklyn|
|Journal||Journal of Physical Chemistry|
|Organization||Polytechnic Inst., Brooklyn|
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