Thumbnail
Access Restriction
Open

Author Lewis, F. A. ♦ Leef, J. L.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ BASIC BIOLOGICAL SCIENCES ♦ APPLIED LIFE SCIENCES ♦ SCHISTOSOMA ♦ FREEZING ♦ INFECTIVITY ♦ RADIOPRESERVATION ♦ VACCINES ♦ CHEMICAL PREPARATION ♦ TESTING ♦ CESIUM 137 ♦ COBALT 60 ♦ CRYOBIOLOGY ♦ INOCULATION ♦ MICE ♦ SCHISTOSOMIASIS ♦ ALKALI METAL ISOTOPES ♦ ANIMALS ♦ BETA DECAY RADIOISOTOPES ♦ BETA-MINUS DECAY RADIOISOTOPES ♦ BIOLOGY ♦ CESIUM ISOTOPES ♦ COBALT ISOTOPES ♦ DISEASES ♦ HELMINTHS ♦ INFECTIOUS DISEASES ♦ INTERMEDIATE MASS NUCLEI ♦ INTERNAL CONVERSION RADIOISOTOPES ♦ IRRADIATION ♦ ISOMERIC TRANSITION ISOTOPES ♦ ISOTOPES ♦ MAMMALS ♦ MINUTES LIVING RADIOISOTOPES ♦ NUCLEI ♦ ODD-EVEN NUCLEI ♦ ODD-ODD NUCLEI ♦ PARASITIC DISEASES ♦ PLATYHELMINTHS ♦ PRESERVATION ♦ RADIOISOTOPES ♦ RODENTS ♦ SYNTHESIS ♦ TREMATODES ♦ VERTEBRATES ♦ YEARS LIVING RADIOISOTOPES 560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987) ♦ Physiological Systems ♦ Radiation SourcesUse in Medical Supply Sterilization(-1987)
Abstract Protection against a Schistosoma mansoni cercarial challenge was evaluated in mice immunized with a vaccine composed of 10-krad-irradiated, cryopreserved schistosomules. The level of resistance induced in C57B1/6 or NMRI (CV) mice increased with the number of schistosomules injected. Up to 83% reduction in challenge worm burden was achieved when 5000 schistosomules were injected per mouse. Intramuscular injection of the vaccine was superior to subcutaneous. Multiple immunizations, up to 3 at 4-week intervals, did not increase the resistance induced by a single immunization. A high level of protection developed in as little as 2 weeks and was maintained through at least 12 weeks postimmunization. The vaccine irradiated with 10 krad from either a 60-cobalt or 137-cesium source induced equivalent levels of resistance, and no differences were found in the immunogenicity of vaccines comprised of organisms irradiated as cercariae or as 1- to 3-hr-old schistosomules. These findings are basic to the development of a cryopreserved, live vaccine against schistosomiasis of humans or domestic animals.
Educational Use Research
Learning Resource Type Article
Publisher Date 1984-06-01
Publisher Place United States
Journal Exp. Parasitol.
Volume Number 57
Issue Number 3
Organization Biomedical Research Inst., Rockville, MD


Open content in new tab

   Open content in new tab