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Author Morishima, Hajime ♦ Sawa, Tsutomu ♦ Takita, Tomohisa ♦ Aoyagi, Takaaki ♦ Takeuchi, Tomio ♦ Umezawa, Hamao
Source J-STAGE
Content type Text
Publisher JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
Language English
Abstract Biosynthesis of pepstatin by $_{Streptomyces$ testaceus} HAMADA $_{et}$ OKAMI was $studied.U-^{14}C-L-Alanine$ and $U-^{14}C-L-valine$ were incorporated into alanine and valine moietiesof pepstatin respectively. $U-^{14}C-L-Leucine,$ $U-^{14}C-malonate$ and $U-^{14}C-acetate$ were incorporatedinto (3S, 4S)-4-amino-3-hydroxy-6-methylheptanoic acid (AHMHA) moiety of pepstatin. Radioactivity of $U-^{14}C-L-leucine$ was located almost exclusively in $C_{3}-C_{8}$ of AHMHA but not in $C_{1}$ and $C_{2}$ and radioactivity of $U-^{14}C-malonate$ was located in $C_{1}$ and $C_{2}$ Labeled AHMHA itself was not incorporated into pepstatin. Malonate was incorporated into pepstatin at about 18 times higher rate than acetate. These results indicate that AHMHA is biosynthesized from L-leucine and malonic acid during biosynthesis of pepstatin and suggested that pepstatin biosynthesis proceeds on a multienzyme system in a similar way as in the case of fatty acid synthesis.
ISSN 00218820
Learning Resource Type Article
Publisher Date 1974-01-01
e-ISSN 18811469
Journal The Journal of Antibiotics(antibiotics1968)
Volume Number 27
Issue Number 4
Page Count 7
Starting Page 267
Ending Page 273


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