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Author Hirai, Keiji ♦ Iyobe, Shizuko ♦ Mitsuhashi, Susumu
Source J-STAGE
Content type Text
Publisher JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
Language English
Abstract A plasmid, Rms425, mediating resistance to streptomycin(Sm) and mercury(Hg) was isolated from Pseudomonas cepacia GN11131 of clinical origin. Rms425 was transferred to Pseudomonas cepacia, Pseudomonas aeruginosa and Escherichia coli strains by transformation with purified DNA and by conjugation between isogenic strains of P. aeruginosa or of E. coil by mixing on membrane filters. The molecular weight of Rms425 was estimated to be about 32 megadaltons by electron microscopy and it was classified as incompatibility group P. Examining the incorporation of $[γ-^{32}P]$ or $[8-^{14}C]$ from isotope-labelled ATP into Sm using the cell-free extracts from P. cepacia or E. coli strains harboring Rms425, we found that the Sm resistance conferred by Rms425 was due to the phosphorylation of the drug.
ISSN 00218820
Learning Resource Type Article
Publisher Date 1982-01-01
e-ISSN 18811469
Journal The Journal of Antibiotics(antibiotics1968)
Volume Number 35
Issue Number 10
Page Count 6
Starting Page 1374
Ending Page 1379


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