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Author Afonso, Adriano ♦ Hon, Frank ♦ Brambilla, Ray
Source J-STAGE
Content type Text
Publisher JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
Language English
Abstract A novel bacterium designated as Aeromonas sp. W-10 produces the antibiotic W-10 complex which comprises of two major and several minor components. The two major components from this complex, Sch 20562 (1) and Sch 20561 (1a), are of biological interest in view of their potent antifungal activity. The chemical degradation studies utilized for the assignment of structure 1 for Sch 20562 are described here. Some of the noteworthy diversity of structural features in this glucosidic cyclic dehydrononapeptide lactone 1 are: an N-terminal (D)-β-hydroxymyristyl unit, three D-amino acid units, two (E)-α-aminocrotonyl units, and an O-α-D-glucosyl-N-methyl-L-allo-threonine unit. The structure determination of 1 utilized the selective cleavage of the dehydropeptide units by ozonolysis to form fragments that were sequenced by mass spectrometry. The stereochemistry of the amino acid units were assigned by isolation of the free amino acids from the hydrolysates of the fragments. The stereochemistry of the α-aminocrotonyl units and the glucosidic linkage were assigned by nmr spectroscopy and molecular rotation data.
ISSN 00218820
Learning Resource Type Article
Publisher Date 1999-04-25
e-ISSN 18811469
Journal The Journal of Antibiotics(antibiotics1968)
Volume Number 52
Issue Number 4
Page Count 15
Starting Page 383
Ending Page 397


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