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Author Ponte Sucre, Alicia ♦ Mendoza León, Alexis ♦ Moll, Heidrun
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2001
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract We have previously shown that cultured Leishmania promastigotes are sensitive to drugs blocking K+ and Na+ channels and Na+/H+ transport systems and that the percentage of parasite-infected macrophages decreases significantly in the presence of the drugs. In the present work, we analyzed whether this drug susceptibility of intracellular amastigotes was associated with the activation of macrophage microbicidal mechanisms. Pretreatment of the cells with glibenclamide (GLIB) increased their resistance to infection with Leishmania, an effect that may be mediated by calcium fluxes since it was reversed by ethylene glycol bis-(β-aminoethyl ether)-N,N,N′,N-tetraacetic acid (EGTA). It was noteworthy that in infected macrophages posttreated with the drugs the clearance of parasites was strongly enhanced when the cells were treated simultaneously with GLIB and interferon-γ; this effect correlated with an increased production of reactive nitrogen intermediates. In conclusion, the data suggest that GLIB treatment increases the resistance of macrophages to infection with Leishmania and potentiates the interferon-γ-stimulated clearance of parasites via the induction of nitric oxide.
ISSN 09320113
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2001-01-01
Publisher Place Berlin/Heidelberg
e-ISSN 14321955
Journal Parasitology Research
Volume Number 87
Issue Number 1
Page Count 5
Starting Page 27
Ending Page 31


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Source: SpringerLink