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Author Rodriguez, Patricia ♦ Carlier, Yves ♦ Truyens, Carine
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2011
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Neonate ♦ Trypanosoma cruzi ♦ Parasite ♦ Dendritic cell ♦ Co-stimulation ♦ T cell proliferation ♦ IFN-γ ♦ Medical Microbiology ♦ Immunology
Abstract We previously reported that Trypanosoma cruzi, the agent of Chagas disease, induces in congenitally infected fetuses a strong, adult-like parasite-specific CD8+ T cell response producing IFN-γ (Hermann et al. in Blood 100:2153–2158, 2002). This suggests that the parasite is able to overcome the immaturity of neonatal antigen presenting cells, an issue which has not been previously addressed. We therefore investigated in vitro the ability of T. cruzi to activate cord blood DCs and compared its effect to that on adult cells. We show that T. cruzi induces phenotypic maturation of cord blood CD11c+ myeloid DCs (mDCs), by enhancing surface expression of CD40, CD80, and CD83, and that parasite-specific IgG purified from cord blood of neonates born to T. cruzi-infected mothers amplify such expression. CD83, considered as the best marker of mature DCs, reaches higher level on cord blood than on adult mDCs. Allo-stimulation experiments showed that T. cruzi-activated cord blood mononuclear cells enriched in DCs (eDCs) stimulate proliferation of cord blood and adult CD3+ T cells to a similar extent. Of note, T. cruzi-activated eDCs from cord blood trigger more potent proliferation of CD8+ than CD8− (mainly CD4+) adult T cells, a feature not observed with adult eDCs. T cell proliferation is associated with IFN-γ release and down-regulation of IL-13 production. These data show that T. cruzi potently activates human cord blood mDCs and endows eDCs to trigger CD8+ T cell proliferation and favor type 1 immune response. Interestingly, maternal antibodies can strengthen the development of mature DCs that might contribute to overcome the immunological immaturity associated with early life.
ISSN 03008584
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-10-29
Publisher Place Berlin/Heidelberg
e-ISSN 14321831
Journal Medical Microbiology and Immunology
Volume Number 201
Issue Number 2
Page Count 13
Starting Page 157
Ending Page 169


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Source: SpringerLink