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Author Horita, Mitsugu ♦ Morohashi, Hitomi ♦ Komai, Fumiri
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2002
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily (Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant the plantlets to soil in pots without acclimatization, and the plantlets grew in a greenhouse until flowering 2 years later.
ISSN 00320935
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2002-07-26
Publisher Place Berlin/Heidelberg
e-ISSN 14322048
Journal Planta
Volume Number 215
Issue Number 5
Page Count 5
Starting Page 880
Ending Page 884


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Source: SpringerLink