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Author Stratikopoulos, E. E. ♦ Augustis, A. A. ♦ Pavlopoulos, I. D. ♦ Ecomou, K. Ph. ♦ Mintzas, A. ♦ Mathiopoulos, K. D. ♦ Zacharopoulou, Antigone
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2009
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Ceratitis capitata ♦ Medfly ♦ Tephritidae ♦ Microsatellites ♦ In situ hybridization ♦ Cross-species amplification ♦ Animal Genetics and Genomics ♦ Microbial Genetics and Genomics ♦ Cell Biology ♦ Plant Genetics & Genomics ♦ Biochemistry
Abstract The Mediterranean fruit fly, Ceratitis capitata, is a pest of major economic importance and has become a model for the development of SIT control programs for insect pests. Significant information has been accumulated on classical and population genetics of this species during the past 2 decades. However, the availability of molecular markers is limited. Here, we present the isolation and characterization of 159 microsatellite clones and the development of 108 polymorphic microsatellite markers for this insect pest. Mapping by in situ hybridization to polytene chromosomes of 21 microsatellite clones enriched the cytogenetic map that was previously constructed by our group. The enriched map provides a large number of STSs for future genome mapping projects. Cross-species amplification of these microsatellite loci in 12 Tephritidae species and sequence analysis of several amplification products indicated a varying degree of transferability and their possible usefulness as molecular and genetic markers in these species where genetic and molecular tools are limited.
ISSN 16174615
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-06-21
Publisher Place Berlin/Heidelberg
e-ISSN 16174623
Journal Molecular and General Genetics MGG
Volume Number 282
Issue Number 3
Page Count 24
Starting Page 283
Ending Page 306


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Source: SpringerLink