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Author Rubinelli, Peter ♦ Siripornadulsil, Surasak ♦ Gao Rubinelli, Fei ♦ Sayre, Richard T.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2002
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract Early transcriptional responses of a cell wall-deficient mutant of the green alga Chlamydomonas reinhardtii to heavy-metal stress have been investigated using the method of mRNA differential display. We have identified, sequenced, and quantified the induction of a number of transcripts that are up-regulated by a brief (2-h) exposure to 25 µm cadmium chloride, including one transcript which is also highly responsive to iron (Fe) deficiency. These transcripts represent both nuclear- and chloroplast-encoded genes, and include both novel genes and genes with known or suspected functions. Among these is a gene with significant homology to HCR1, a high-CO2- and Fe-deficiency-inducible gene from Chlorococcum littorale. We further characterized the regulation of the HCR1-like gene (H43) and found that this transcript is also induced by Fe-depletion of the medium. Heterologous expression of H43 in the Fe-uptake mutant fet3fet4 of Saccharomyces cerevisiae resulted in partial suppression of the slow-growth phenotype of this mutant in minimal medium, and resulted in a 2-fold increase in Fe accumulation per cell. Our results demonstrate the utility of Chlamydomonas cw – strains for functional genomics studies of metal stress. The magnitudes of induction and functional analyses suggest possible utility for these genes in the study of metal stress sensing in green plants and development of novel Fe acquisition and phytoremediation strategies.
ISSN 00320935
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2002-01-25
Publisher Place Berlin/Heidelberg
e-ISSN 14322048
Journal Planta
Volume Number 215
Issue Number 1
Page Count 13
Starting Page 1
Ending Page 13


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Source: SpringerLink