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Author Kemp, J. T. ♦ Balasubramanian, M. K. ♦ Gould, K. L.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©1997
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract The organization of the actin cytoskeleton plays an integral role in cell morphogenesis of all eukaryotes. We have isolated a temperature-sensitive mutant in Schizosaccharomyces pombe, wat1-1, in which acting patches are delocalized, resulting in an elliptically shaped cell phenotype. Molecular cloning and DNA sequencing of wat1 + showed that the gene encodes a 314 residue protein containing WD-40 repeats. Cells lacking wat1 + are slow growing but viable at 25° C and temperature-sensitive for growth above 33° C. At restrictive temperature, wat1-d strains are phenotypically indistinguishable from wat1-1. When combined with a deletion for the wat1 + gene, cdc mutants failed to elongate at restrictive temperature and exhibited alterations in actin patch localization. This analysis suggests that wat1 + is required directly or indirectly for polarized cell growth in S. pombe. Wat1p and a functional, epitope-tagged, version of Wat1p can be overproduced without inducing alterations in cell morphology.
ISSN 00268925
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1997-03-26
Publisher Place Berlin/Heidelberg
e-ISSN 14321874
Journal Molecular and General Genetics MGG
Volume Number 254
Issue Number 2
Page Count 12
Starting Page 127
Ending Page 138


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Source: SpringerLink