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Author Gemoll, Timo ♦ Habermann, Jens K. ♦ Lahmann, Johanna ♦ Szymczak, Silke ♦ Lundgren, Caroline ♦ Bündgen, Nana K. ♦ Jungbluth, Thomas ♦ rdström, Britta ♦ Becker, Susanne ♦ Lomnytska, Marta I. ♦ Bruch, Hans Peter ♦ Ziegler, Andreas ♦ Hellman, Ulf ♦ Auer, Gert ♦ Roblick, Uwe J. ♦ Jörnvall, Hans
Source SpringerLink
Content type Text
Publisher SP Birkhäuser Verlag Basel
File Format PDF
Copyright Year ©2011
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Aneuploidy ♦ Endometrial carcinoma ♦ Genomic instability ♦ Mass spectrometry ♦ Pathway analysis ♦ Two-dimensional gel electrophoresis ♦ Biochemistry ♦ Cell Biology ♦ Life Sciences ♦ Biomedicine general
Abstract DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n = 5), diploid (n = 7), and aneuploid (n = 7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-κB as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer.
ISSN 1420682X
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-07-08
Publisher Place Basel
e-ISSN 14209071
Journal Cellular and Molecular Life Sciences
Volume Number 69
Issue Number 2
Page Count 9
Starting Page 325
Ending Page 333


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Source: SpringerLink