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Author Graichen, Heiko ♦ Lochmüller, Eva Maria ♦ Wolf, Eckhard ♦ Langkabel, Bernd ♦ Stammberger, Tobias ♦ Haubner, Michael ♦ Renner Müller, Ingrid ♦ Englmeier, Karl Hans ♦ Eckstein, F.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©1999
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract  A non-destructive, three-dimensional technique for microstructural phenotypic characterisation of skeletal elements in genetically altered mice is presented. Preliminary data in bovine growth-hormone transgenic animals and control littermates are shown. The technique is based on microcomputed tomography (μCT) and digital postprocessing and allows for a differential quantitative analysis of the cortical and trabecular bone compartments in the axial and peripheral skeleton. The distal femora and the first lumbar vertebral bodies of six animals were CT scanned in the axial plane with an isotropic resolution of 20 µm. The periostal surface and the marrow spaces were segmented fully automatically, and the trabecular and cortical compartments were separated interactively. After 3-D reconstruction, various regions of interest (diaphyseal, metaphyseal and epiphyseal) were selected for the analysis. The femora and vertebrae of the transgenic animals showed obvious differences in size, shape, and trabecular arrangement compared with the control animals. The total bone mass was increased by a factor of two to three, but the trabecular bone was increased much more (up to 12 times) than the cortical bone. The transgenic animals showed an increased ratio of trabecular vs cortical bone (0.90 to 1.27 vs 0.14 to 0.36 in the femoral diaphysis) and an elevated trabecular bone volume fraction (49% to 73% vs 18% to 43% in the femoral metaphysis). The mean 3-D cortical thickness was similar in the normal and transgenic animals (values between 93 µm and 232 µm in the dia- and metaphyses), but the minimal cortical thickness was lower in the transgenic animals (22 to 31 µm vs 54 µm to 110 µm in the diaphysis). The technique presented is suitable for phenotypic characterisation of bone structure in genetically altered mice.
ISSN 03402061
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1999-01-28
Publisher Place Berlin/Heidelberg
e-ISSN 14320568
Journal Anatomy and Embryology
Volume Number 199
Issue Number 3
Page Count 10
Starting Page 239
Ending Page 248


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Source: SpringerLink