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Author Raggam, R. ♦ Leitner, E. ♦ Mühlbauer, G. ♦ Berg, J. ♦ Stöcher, M. ♦ Grisold, A. ♦ Marth, E. ♦ Kessler, H.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2002
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract Molecular assays for qualitative detection of Legionella spp. in clinical specimens were evaluated. DNA extraction was done either with a fully automated DNA extraction protocol on the MagNA Pure LC System or with manual DNA extraction. Amplification and detection were done by real-time polymerase chain reaction (PCR) on the LightCycler (LC) instrument. Oligonucleotides were derived from the 16S rRNA gene of Legionella spp. The assays included a specially designed DNA fragment as Legionella-specific internal control. For both molecular assays, the detection limit was determined to be 5 CFU per LC PCR run. Sixty-one clinical specimens were tested with the molecular assays. Results were compared to culture. Five samples were found to be positive with the molecular assays. Three of them were positive in culture. No inhibition was found throughout the whole study. In conclusion, the molecular assays described may lead to safe and early diagnosis of Legionnaires' disease. They proved to be suitable for the routine molecular diagnostics laboratory.
ISSN 03008584
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2002-08-29
Publisher Place Berlin/Heidelberg
e-ISSN 14321831
Journal Medical Microbiology and Immunology
Volume Number 191
Issue Number 2
Page Count 7
Starting Page 119
Ending Page 125


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Source: SpringerLink