### Sesqui-Ds, the chromosome-breaking insertion at bz-m1, links double Ds to the original Ds elementSesqui-Ds, the chromosome-breaking insertion at bz-m1, links double Ds to the original Ds element

Access Restriction
Subscribed

 Author Martínez Férez, I. M. ♦ Dooner, H. K. Source SpringerLink Content type Text Publisher Springer-Verlag File Format PDF Copyright Year ©1997 Language English
 Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology Abstract The bz-m1 mutation in maize was one of the first to arise by direct transposition of the chromosome-breaking Ds element from its original or standard' location in chromosome 9S to a known locus in the same chromosome arm. Thus, elucidation of its structure should shed light on the nature of the original Ds element described by McClintock in 1948. The Ds insertion in bz-m1 has been reported to be only 1.2 kb long – much shorter than other chromosome-breaking Ds elements that have been described. We have characterized here the Ds element in our bz-m1 stocks and have confirmed by genetic and molecular tests that, in the presence of Ac, it acts as a chromosome breaker. The Ds insertion at bz-m1 is 1260 bp long. Besides its normal 5′ and 3′ ends, it contains an internal 3′ end at the same junction as the chromosome-breaking double Ds element that has been found in several sh mutations. Thus, it appears to have arisen from the 4.1-kb double Ds by internal deletion of 2.9 kb. Because the element has lost one internal 5′ end, but retains the chromosome-breaking properties of double Ds, we have named it sesqui-Ds (sDs). The origin, structure and properties of sDs vis-à-vis double Ds support the hypothesis that double Ds corresponds to the chromosome-breaking Ds element at the standard' location in 9S. ISSN 00268925 Age Range 18 to 22 years ♦ above 22 year Educational Use Research Education Level UG and PG Learning Resource Type Article Publisher Date 1997-08-28 Publisher Place Berlin/Heidelberg e-ISSN 14321874 Journal Molecular and General Genetics MGG Volume Number 255 Issue Number 6 Page Count 7 Starting Page 580 Ending Page 586