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Author Fampa, Patrícia ♦ Santos, André L. S. ♦ Ramirez, Marcel I.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2010
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Immunology ♦ Microbiology ♦ Medical Microbiology
Abstract Trypanosoma cruzi presents considerable genetic and protein profile polymorphism among different parasite populations. In a previous work, our group indicated cysteine and metalloprotease activities as good markers for separating T. cruzi I (TCI) from T. cruzi II (TCII) isolates, with higher heterogeneity among TCII isolates. Here, we have investigated the expression level of surface cruzipain in 16 field isolates belonging to the genotype TCI (n = 8) and TCII (n = 8) of T. cruzi. By means of flow cytometry analyses, using an anti-cruzipain polyclonal antibody, we observed a highly heterogeneous pattern of surface cruzipain molecules in these isolates, independently of their genotypes, cell measurements (size and granularity), original hosts, or biomes. However, fluorescence labeling tended to be stronger in TCI than in TCII population. Interestingly, isolates that expressed higher levels of surface cruzipain also yielded elevated levels of metacyclogenesis in vitro.
ISSN 09320113
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-05-30
Publisher Place Berlin/Heidelberg
e-ISSN 14321955
Journal Parasitology Research
Volume Number 107
Issue Number 2
Page Count 5
Starting Page 443
Ending Page 447


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Source: SpringerLink