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Author Kawai Yamada, Maki ♦ Naga, Miru ♦ Kakimoto, Masayuki ♦ Uchimiya, Hirofumi
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2013
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Arabidopsis ♦ Embryogenesis ♦ Lethal ♦ Mutant ♦ Plant Sciences ♦ Agriculture ♦ Ecology ♦ Forestry
Abstract Arabidopsis cell growth defect factor-1 (Cdf1 in yeast, At5g23040) was originally isolated as a cell growth suppressor of yeast from genetic screening. To investigate the in vivo role of Cdf1 in plants, a T-DNA insertion line was analyzed. A homozygous T-DNA insertion mutant (cdf1/cdf1) was embryo lethal and showed arrested embryogenesis at the globular stage. The Cdf1 protein, when fused with green fluorescent protein, was localized to the plastid in stomatal guard cells and mesophyll cells. A promoter-β-glucuronidase assay found expression of Cdf1 in the early heart stage of embryogenesis, suggesting that Cdf1 was essential for Arabidopsis embryogenesis during the transition of the embryo from the globular to heart stage.
ISSN 00320935
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-10-06
Publisher Place Berlin/Heidelberg
e-ISSN 14322048
Journal Planta
Volume Number 239
Issue Number 1
Page Count 8
Starting Page 39
Ending Page 46

Source: SpringerLink