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Author Mohamed, Walid ♦ Darji, Ayub ♦ Domann, Eugen ♦ Chiancone, Emilia ♦ Chakraborty, Trinad
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2006
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Animal Genetics and Genomics ♦ Plant Genetics & Genomics ♦ Microbial Genetics and Genomics ♦ Biochemistry ♦ Cell Biology
Abstract The identity and role of listerial antigens recognized by antibodies following listerial infection is largely unknown. We identified the listerial ferritin-like protein Frm as a target of the humoral response following infection of mice with pathogenic Listeria monocytogenes. Specific antibodies to Frm are detected in antisera of mice infected with a pathogenic strain but not in antisera of mice infected with a non-pathogenic L. innocua strain. Antibodies raised to purified listerial ferritin allowed us to demonstrate that the expression of Frm is both growth phase- and temperature-dependent. Using an isogenic Δfrm mutant, we find that ferritin is essential for bacterial growth in chemically defined minimal media but not in complex media such as brain–heart infusion. Mutant bacteria also exhibit a defect in intracellular replication. The Δfrm strain is hypersensitive to hydrogen peroxide indicating that the Frm is required for protection against reactive oxygen intermediates under various growth conditions. Animal studies show that Frm contributes to pathogenesis in mice, in particular, at early time points following infection.
ISSN 16174615
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2006-03-10
Publisher Place Berlin/Heidelberg
e-ISSN 16174623
Journal Molecular and General Genetics MGG
Volume Number 275
Issue Number 4
Page Count 10
Starting Page 344
Ending Page 353


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Source: SpringerLink