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Author Primikirios, Nikolas I. ♦ Roubelakis Angelakis, Kalliopi A.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©1999
Language English
Subject Domain (in DDC) Social sciences ♦ Sociology & anthropology
Abstract Arginine decarboxylase (ADC; EC 4.1.1.19) is a key enzyme in one of the two pathways to putrescine. We present the first ADC cDNA from a woody perennial plant species, the grapevine (Vitis vinifera L.), which exhibits 70–80% homology with other dicot ADCs. The effects of ammonium, nitrate, and putrescine on ADC specific activity, soluble polyamine levels, ADC-mRNA, endogenous arginine and ornithine, and arginase specific activity were investigated in suspension cultures of grapevine cells. The addition of NH+ 4 to cells cultured in NH+ 4-free medium, resulted in a 4-fold increase in ADC activity and concomitantly in a 4-fold increase in putrescine and a 3-fold decrease in arginine. During this period ornithine increased and arginase activity followed a reverse pattern of changes compared with ADC. In contrast, the addition of NO− 3 did not markedly affect ADC activity, putrescine, arginine and ornithine, but transiently increased arginase activity. The addition of putrescine caused a 4-fold decrease in ADC activity and increased arginine, ornithine and arginase activity. The changes in ADC specific activity were not accompanied by analogous changes in the ADC transcript levels. These results further support the view that ADC regulation is not exhibited, at least for the factors considered in this work, at the transcriptional level.
ISSN 00320935
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1999-06-15
Publisher Place Berlin/Heidelberg
e-ISSN 14322048
Journal Planta
Volume Number 208
Issue Number 4
Page Count 9
Starting Page 574
Ending Page 582


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Source: SpringerLink