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Author Shao, Yu Feng ♦ Wang, Can ♦ Xie, Jun Fan ♦ Kong, Xiang Pan ♦ Xin, Le ♦ Dong, Chao Yu ♦ Li, Jing ♦ Ren, Wen Ting ♦ Hou, Yi Ping
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2015
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Neuropeptide S (NPS) ♦ Neuropeptide S receptor (NPSR) ♦ Olfactory spatial memory ♦ Subiculum complex ♦ Muscarinic cholinergic receptor ♦ N-methyl-d-aspartate receptor ♦ Antagonist ♦ c-Fos ♦ Neurosciences ♦ Cell Biology ♦ Neurology
Abstract Our previous studies have demonstrated that neuropeptide S (NPS), via selective activation of the neurons bearing NPS receptor (NPSR) in the olfactory cortex, facilitates olfactory function. High level expression of NPSR mRNA in the subiculum complex of hippocampal formation suggests that NPS–NPSR system might be involved in the regulation of olfactory spatial memory. The present study was undertaken to investigate effects of NPS on the scopolamine- or MK801-induced impairment of olfactory spatial memory using computer-assisted 4-hole-board spatial memory test, and by monitoring Fos expression in the subiculum complex in mice. In addition, dual-immunofluorescence microscopy was employed to identify NPS-induced Fos-immunereactive (-ir) neurons that also bear NPSR. Intracerebroventricular administration of NPS (0.5 nmol) significantly increased the number of visits to switched odorants in recall trial in mice suffering from odor-discriminating inability induced by scopolamine, a selective muscarinic cholinergic receptor antagonist, or MK801, a N-methyl-d-aspartate receptor antagonist, after training trials. The improvement of olfactory spatial memory by NPS was abolished by the NPSR antagonist [D-Val5]NPS (40 nmol). Ex vivo c-Fos and NPSR immunohistochemistry revealed that, as compared with vehicle-treated mice, NPS markedly enhanced Fos expression in the subiculum complex encompassing the subiculum (S), presubiculum (PrS) and parasubiculum (PaS). The percentages of Fos-ir neurons that also express NPSR were 91.3, 86.5 and 90.0 % in the S, PrS and PaS, respectively. The present findings demonstrate that NPS, via selective activation of the neurons bearing NPSR in the subiculum complex, ameliorates olfactory spatial memory impairment induced by scopolamine and MK801 in mice.
ISSN 18632653
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-09-01
Publisher Place Berlin/Heidelberg
e-ISSN 18632661
Journal Anatomy and Embryology
Volume Number 221
Issue Number 6
Page Count 10
Starting Page 3327
Ending Page 3336


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Source: SpringerLink