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Author Kimura, Akio ♦ Edagawa, Akiko ♦ Okada, Kunihiro ♦ Takimoto, Atsutoshi ♦ Yonesho, Shizuo ♦ Karanis, Panagiotis
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2007
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Immunology ♦ Microbiology ♦ Medical Microbiology
Abstract We investigated the prevalence and genotypes of Cryptosporidium parasite in 50 brown rats (Rattus norvegicus) inhabiting an urban area of Japan. Fecal samples collected from the animals were examined by an immuno-fluorescence assay (IFA). Genomic DNA was extracted directly from fecal sample of each animal and nested PCR was performed to amplify part of the 18S ribosomal RNA (18SrRNA) of the Cryptosporidium species. The detection rate was 8% by IFA and 38% by nested PCR. The sequence and phylogenetic analyses of 13 PCR products showed that the Cryptosporidium from brown rats were clustered into four distinct genotypes. Interestingly, one of the four genotypes was significantly distinct from the C. parvum and C. hominis genotypes. Our results suggest the existence of a new genotype of Cryptosporidium in brown rats.
ISSN 09320113
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-02-21
Publisher Place Berlin/Heidelberg
e-ISSN 14321955
Journal Parasitology Research
Volume Number 100
Issue Number 6
Page Count 4
Starting Page 1417
Ending Page 1420


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Source: SpringerLink