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Author Pannell, Maria ♦ Meier, Maria Almut ♦ Szulzewsky, Frank ♦ Matyash, Vitali ♦ Endres, Matthias ♦ Kronenberg, Golo ♦ Prinz, Vincent ♦ Waiczies, Sonia ♦ Wolf, Susanne A. ♦ Kettenmann, Helmut
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2014
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Muscarinic acetylcholine receptors ♦ Microglia ♦ Brain macrophages ♦ Stroke ♦ Alzheimer’s disease ♦ Glioma ♦ Multiple sclerosis ♦ Neurosciences ♦ Cell Biology ♦ Neurology
Abstract Microglia undergo a process of activation in pathology which is controlled by many factors including neurotransmitters. We found that a subpopulation (11 %) of freshly isolated adult microglia respond to the muscarinic acetylcholine receptor agonist carbachol with a Ca2+ increase and a subpopulation of similar size (16 %) was observed by FACS analysis using an antibody against the M3 receptor subtype. The carbachol-sensitive population increased in microglia/brain macrophages isolated from tissue of mouse models for stroke (60 %) and Alzheimer’s disease (25 %), but not for glioma and multiple sclerosis. Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-γ to around 60 %. This increase was sensitive to blockers of protein synthesis and correlated with an upregulation of the M3 receptor subtype and with an increased expression of MHC-I and MHC-II. Carbachol was a chemoattractant for microglia and decreased their phagocytic activity.
ISSN 18632653
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-12-19
Publisher Place Berlin/Heidelberg
e-ISSN 18632661
Journal Anatomy and Embryology
Volume Number 221
Issue Number 2
Page Count 16
Starting Page 1157
Ending Page 1172


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Source: SpringerLink