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Author Ribarits, Alexandra ♦ Abdullaev, Alisher ♦ Tashpulatov, Alisher ♦ Richter, Andreas ♦ Heberle Bors, Erwin ♦ Touraev, Alisher
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2006
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Arabidopsis ♦ Development ♦ Nicotiana ♦ Proline dehydrogenase ♦ RNA interference (RNAi) ♦ Stress ♦ Forestry ♦ Ecology ♦ Agriculture ♦ Plant Sciences
Abstract Proline dehydrogenase is the rate-limiting enzyme in proline degradation and serves important functions in the stress responses and development of plants. We isolated two tobacco proline dehydrogenases, NtPDH1 and NtPDH2, in the course of screening for genes upregulated in stressed tobacco (Nicotiana tabacum) microspores. Expression analysis revealed that the two genes are differentially regulated. Under unstressed conditions, their steady-state transcript levels were similar in mature pollen and apical meristems, whereas NtPDH2 was expressed predominantly in vegetative organs, styles, and ovules. The expression of NtPDH1 was maintained at a constant low level during 24 h of dehydration, whereas NtPDH2 was upregulated within 1 h after the onset of stress and subsequently downregulated to undetectable levels. Differential and sustained expression was also found for the two enzymatic isoforms of Arabidopsis thaliana AtPDH. Silencing of the NtPDH genes by RNA interference using the CaMV 35S promoter led to increased proline contents, decreased seed set, delayed seed germination and retarded seedling development pointing towards an important function of at least one of the two NtPDH genes during plant reproductive development.
ISSN 00320935
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2006-11-15
Publisher Place Berlin/Heidelberg
e-ISSN 14322048
Journal Planta
Volume Number 225
Issue Number 5
Page Count 12
Starting Page 1313
Ending Page 1324


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Source: SpringerLink