Thumbnail
Access Restriction
Open

Author Nagle, Peter W. ♦ Hosper, Nynke A. ♦ Ploeg, Emily M. ♦ Goethem, Marc-Jan van ♦ Brandenburg, Sytze ♦ Langendijk, Johannes A. ♦ Chiu, Roland K. ♦ Coppes, Robert P.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIOLOGY AND NUCLEAR MEDICINE ♦ ANIMAL TISSUES ♦ CARBON IONS ♦ DNA REPAIR ♦ IN VITRO ♦ IRRADIATION ♦ KEV RANGE 10-100 ♦ LET ♦ PLANT TISSUES ♦ RADIOTHERAPY ♦ SALIVARY GLANDS ♦ STEM CELLS ♦ STRAND BREAKS
Abstract Purpose: A reduction in the dose, irradiated volume, and sensitivity of, in particular, normal tissue stem cells is needed to advance radiation therapy. This could be obtained with the use of particles for radiation therapy. However, the radiation response of normal tissue stem cells is still an enigma. Therefore, in the present study, we developed a model to investigate the in vitro response of stem cells to particle irradiation. Methods and Materials: We used the immortalized human salivary gland (HSG) cell line resembling salivary gland (SG) cells to translate the radiation response in 2-dimensional (2D) to 3-dimensional (3D) conditions. This response was subsequently translated to the response of SG stem cells (SGSCs). Dispersed single cells were irradiated with photons or carbon ions at different linear energy transfers (LETs; 48.76 ± 2.16, 149.9 ± 10.8, and 189 ± 15 keV/μm). Subsequently, 2D or 3D clonogenicity was determined by counting the colonies or secondary stem cell-derived spheres in Matrigel. γH2AX immunostaining was used to assess DNA double strand break repair. Results: The 2D response of HSG cells showed a similar increase in dose response to increasing higher LET irradiation as other cell lines. The 3D response of HSG cells to increasing LET irradiation was reduced compared with the 2D response. Finally, the response of mouse SGSCs to photons was similar to the 3D response of HSG cells. The response to higher LET irradiation was reduced in the stem cells. Conclusions: Mouse SGSC radiosensitivity seems reduced at higher LET radiation compared with transformed HSG cells. The developed model to assess the radiation response of SGSCs offers novel possibilities to study the radiation response of normal tissue in vitro.
ISSN 03603016
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-01
Publisher Place United States
Journal International Journal of Radiation Oncology, Biology and Physics
Volume Number 95
Issue Number 1


Open content in new tab

   Open content in new tab