Access Restriction

Author Rockwood, Alan L. ♦ Erve, John C. L.
Source SpringerLink
Content type Text
Publisher Springer US
File Format PDF
Copyright Year ©2014
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences
Subject Keyword Fourier transform ♦ Peak distortions ♦ Non-linear ♦ Non-linear operator ♦ Linear operator ♦ FT-ICR ♦ Orbitrap ♦ Isotopic fine structure ♦ Beat pattern ♦ Fourier Transform Mass Spectrometry ♦ FTMS ♦ Analytical Chemistry ♦ Biotechnology ♦ Organic Chemistry ♦ Proteomics ♦ Bioinformatics
Abstract Distortions of peaks can occur when one uses the standard method of signal processing of data from the Orbitrap and other FT-based methods of mass spectrometry. These distortions arise because the standard method of signal processing is not a linear process. If one adds two or more functions, such as time-dependent signals from a Fourier transform mass spectrometer and performs a linear operation on the sum, the result is the same as if the operation was performed on separate functions and the results added. If this relationship is not valid, the operation is non-linear and can produce unexpected and/or distorted results. Although the Fourier transform itself is a linear operator, the standard algorithm for processing spectra in Fourier transform-based methods include non-linear mathematical operators such that spectra processed by the standard algorithm may become distorted. The most serious consequence is that apparent abundances of the peaks in the spectrum may be incorrect. In light of these considerations, we performed theoretical modeling studies to illustrate several distortion effects that can be observed, including abundance distortions. In addition, we discuss experimental systems where these effects may manifest, including suggested systems for study that should demonstrate these peak distortions. Finally, we point to several examples in the literature where peak distortions may be rationalized by the phenomena presented here. Graphical Abstract ᅟ
ISSN 10440305
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-09-27
Publisher Institution The American Society for Mass Spectrometry
Publisher Place Boston
e-ISSN 18791123
Journal Journal of The American Society for Mass Spectrometry
Volume Number 25
Issue Number 12
Page Count 14
Starting Page 2163
Ending Page 2176

Open content in new tab

   Open content in new tab
Source: SpringerLink