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Author Pužar Dominkuš, Pia ♦ Ferdin, Jana ♦ Plemenitaš, Ana ♦ Peterlin, Boris Matija ♦ Lenassi, Metka
Source SpringerLink
Content type Text
Publisher Springer International Publishing
File Format PDF
Copyright Year ©2017
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Astrocytes ♦ HIV-1 ♦ Nef ♦ Extracellular vesicles ♦ Exosomes ♦ Central nervous system ♦ Neurosciences ♦ Virology ♦ Infectious Diseases ♦ Immunology ♦ Neurology
Abstract HIV-1 infection of the central nervous system causes HIV-associated neurocognitive disorders, even in aviremic patients. Although astrocyte malfunction was associated to these disorders, their implication is overshadowed by contributions of microglia and macrophages. Astrocytes are infected with HIV-1 in vivo and express a relevant amount of viral protein Nef. Nef was shown to stimulate its own release in exosomes from diverse cell types, which in turn have damaging effects on neighboring cells. Using immunoblotting and electron microscopy, we showed that human astrocytes expressing Nef.GFP similarly release Nef in exosomes. Importantly, Nef.GFP expression increases the secretion of exosomes from human astrocytes up to 5.5-fold, as determined by total protein content and nanoparticle tracking analysis. Protein analysis of exosomes and viruses separated on iodixanol gradient further showed that native or pseudotyped HIV-1-infected human astrocytes release exosomes, which contain Nef. Our results provide the basis for future studies of the damaging role of Nef-exosomes produced by HIV-infected astrocytes on the central nervous system.
ISSN 13550284
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2017-07-31
Publisher Place Cham, Switzerland
e-ISSN 15382443
Journal Journal of NeuroVirology
Volume Number 23
Issue Number 5
Page Count 12
Starting Page 713
Ending Page 724


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Source: SpringerLink