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Author Meriem, Gasmi ♦ Mahmoud, Kitouni
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2016
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Chitinase activity ♦ Streptomyces griseorubens C9 ♦ Plackett–Burman ♦ Response surface methodology ♦ Box–Wilson ♦ Microbiology ♦ Microbial Genetics and Genomics ♦ Microbial Ecology ♦ Mycology ♦ Medical Microbiology ♦ Applied Microbiology
Abstract The purpose of this article is to use statistical Plackett–Burman and Box–Wilson response surface methodology to optimize the medium components and, thus, improve chitinase production by Streptomyces griseorubens C9. This strain was previously isolated and identified from a semi-arid soil of Laghouat region (Algeria). First, syrup of date, colloidal chitin, yeast extract and K$_{2}$HPO$_{4}$, KH$_{2}$PO$_{4}$ were proved to have significant effects on chitinase activity using the Plackett–Burman design. Then, an optimal medium was obtained by a Box–Wilson factorial design of response surface methodology in liquid culture. Maximum chitinase production was predicted in medium containing 2% colloidal chitin, 0.47% syrup of date, 0.25 g/l yeast extract and 1.81 g/l K$_{2}$HPO$_{4}$, KH$_{2}$PO$_{4}$ using response surface plots of the STATISTICA software v.12.0.
ISSN 15904261
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2016-12-24
Publisher Place Berlin, Heidelberg
e-ISSN 18692044
Journal Annals of Microbiology
Volume Number 67
Issue Number 2
Page Count 9
Starting Page 175
Ending Page 183

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Source: SpringerLink