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Author Pinto Leite, Rosário ♦ Carreira, Isabel ♦ Melo, Joana ♦ Ferreira, Susana Isabel ♦ Ribeiro, Ilda ♦ Ferreira, Jaqueline ♦ Filipe, Marco ♦ Bernardo, Carina ♦ Arantes Rodrigues, Regina ♦ Oliveira, Paula ♦ Santos, Lúcio
Source SpringerLink
Content type Text
Publisher Springer Netherlands
File Format PDF
Copyright Year ©2014
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Urinary bladder cancer ♦ Urinary bladder cancer cell lines ♦ Genomic ♦ Chromosome ♦ aCGH ♦ MLPA ♦ Cancer Research
Abstract Several genomic regions are frequently altered and associated with the type, stage and progression of urinary bladder cancer (UBC). We present the characterization of 5637, T24 and HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative genomic hybridization (aCGH) and multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies present in UBC were found in the three cell lines, such as chromosome 20 aneuploidy and the loss of 9p21. Some gene loci losses (e.g. CDKN2A) and gains (e.g. HRAS, BCL2L1 and PTPN1) were coincident across all cell lines. Although some significant heterogeneity and complexity were detected between them, their genomic profiles exhibited a similar pattern to UBC. We suggest that 5637 and HT1376 represent the E2F3/RB1 pathway due to amplification of 6p22.3, concomitant with loss of one copy of RB1 and mutation of the remaining copy. The HT1376 presented a 10q deletion involving PTEN region and no alteration of PIK3CA region which, in combination with the inactivation of TP53, bears more invasive and metastatic properties than 5637. The T24 belongs to the alternative pathway of FGFR3/CCND1 by presenting mutated HRAS and over-represented CCND1. These cell lines cover the more frequent subtypes of UBC and are reliable models that can be used, as a group, in preclinical studies.
ISSN 10104283
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-01-24
Publisher Place Dordrecht
e-ISSN 14230380
Journal Tumor Biology
Volume Number 35
Issue Number 5
Page Count 19
Starting Page 4599
Ending Page 4617


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Source: SpringerLink