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Author Xu, Bin ♦ Peng, Min ♦ Song, Qibin
Source SpringerLink
Content type Text
Publisher Springer Netherlands
File Format PDF
Copyright Year ©2013
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Quantum dots ♦ Human breast cancer ♦ Her-2 ♦ Telomerase ♦ Alternative lengthening of telomeres ♦ Cancer Research
Abstract Recently, telomerase-targeted therapy was studied intensively; however, many studies have verified the existence of alternative lengthening mechanisms of telomeres in vitro. In the present work, we explored the expression characteristic of the two kinds of telomere-prolonging mechanisms in the breast cancer tissues per se. Furthermore, we studied the relationship between Her-2 expression and ALT pathway. Ninety samples of breast cancer tissues were examined in this research. RT-PCR was used for the detection of the expression of human telomerase reverse transcriptase (hTERT); IHC was used for the detection of the expression of promyelocytic leukemia body (PML) bodies; the co-expression of PML bodies and hTERT was detected using the QDs-based immunofluorescence. The co-expression of PML body and hTERT was found in the same cell in breast cancer tissues, and ten samples expressed neither PML bodies nor hTERT. Additionally, the expression of PML bodies and Her-2 was statistically co-related (P = 0.047). The two kinds of mechanisms of telomere extension can co-exist in the same cell in beast cancer tissues, and there may be other mechanisms of telomere extension functioning in human breast carcinoma.
ISSN 10104283
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-12-28
Publisher Place Dordrecht
e-ISSN 14230380
Journal Tumor Biology
Volume Number 35
Issue Number 5
Page Count 7
Starting Page 4087
Ending Page 4093


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Source: SpringerLink