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Author Li, Mei ♦ Qiu, Lihong ♦ Pang, Yi
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2007
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword cloning ♦ enterotoxin ♦ expression ♦ Photorhabdus ♦ toxicity ♦ toxin gene/plu0840 ♦ Microbiology ♦ Microbial Genetics and Genomics ♦ Microbial Ecology ♦ Fungus Genetics ♦ Medical Microbiology ♦ Applied Microbiology
Abstract The genome of the insect pathogenPhotorhabdus luminescens TT01 strain contains multiple genes predicted to encode toxins. One of these,plu0840, has 55% sequence identity with an enterotoxin fromAeromonas hydrophila. In order to further investigate this gene, we successfully cloned the completeplu0840 fromPhotorhabdus sp. HB78 and expressed it as a GST-Plu0840 fusion protein inEscherichia coli BL21 (DE3) using pGEX-4T-1 as a vector. Most of GST-Plu0840 was insoluble and sequestered into inclusion bodies. The inclusion bodies were harvested and dissolved. The resultant protein was cleaved and purified from the GST-tag. Oral bioassay showed that Plu0840 inhibited growth ofSpodoptera litura andSpodoptera exigua larvae.
ISSN 15904261
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-01-01
Publisher Place Berlin, Heidelberg
e-ISSN 18692044
Journal Annals of Microbiology
Volume Number 57
Issue Number 3
Page Count 7
Starting Page 313
Ending Page 319


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Source: SpringerLink