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Author Ekins, M. G. ♦ Hayden, H. L. ♦ Aitken, E. A. B. ♦ Goulter, K. C.
Source SpringerLink
Content type Text
Publisher Springer Netherlands
File Format PDF
Copyright Year ©2010
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Genetic diversity ♦ genotype ♦ MCG ♦ RAPD ♦ RFLP ♦ Population genetics ♦ Clonal ♦ Homothallic ♦ Asexual ♦ Sexual ♦ Reproduction ♦ Temporal ♦ Head rot ♦ Basal stem rot ♦ Ascospores ♦ Ecology ♦ Plant Sciences ♦ Plant Pathology ♦ Entomology ♦ Agriculture
Abstract Sunflower (Helianthus annuus) is host to infections by Sclerotinia sclerotiorum originating from either homothallic sexually-derived ascospores (stem and head rots) or asexually-derived sclerotia (root rot). While sunflower can be infected by either ascospores or sclerotia this study found no association between the genotypes found in lesions and the type of infection (stem, head or root rot). Multicopy Restriction Fragment Length Polymorphisms (RFLPs) showed individual sclerotia comprised of only one genotype, and that all eight ascospores within an ascus also had only one genotype. Mycelial Compatibility Groups (MCGs), Random Amplified Polymorphic DNAs (RAPDs), single and multicopy RFLP analyses all showed the majority of sunflower plants were infected by only one genotype. A sample of 250 isolates collected hierarchically from sunflowers in Queensland and New South Wales were shown to belong to one large genetic population of S. sclerotiorum. Temporal studies revealed genetic uniformity was maintained across years, further confirming one genetic population. A range of molecular markers were used to genotype 120 isolates, resulting in differing levels of resolution of a genotype. Between 13 and 24 genotypes were identified with similarities and differences in the assemblages of isolates within each genotype depending on the marker used.
ISSN 08153191
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-12-08
Publisher Institution Australasian Plant Pathology Society
Publisher Place Dordrecht
e-ISSN 14486032
Journal Australasian Plant Pathology
Volume Number 40
Issue Number 2
Page Count 10
Starting Page 99
Ending Page 108


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Source: SpringerLink