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Author Munisamy, Sharon M. ♦ Chambliss, C. Kevin ♦ Becker, Christopher
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2012
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences
Subject Keyword Ion mobility-mass spectrometry ♦ Lignocellulomics ♦ Biomass ♦ Carbohydrates ♦ Corn stover ♦ Sorghum ♦ Bioinformatics ♦ Proteomics ♦ Biotechnology ♦ Organic Chemistry ♦ Analytical Chemistry
Abstract Direct infusion electrospray ionization – ion mobility – high resolution mass spectrometry (DIESI-IM-HRMS) has been utilized as a rapid technique for the characterization of total molecular composition in “whole-sample” biomass hydrolysates and extracts. IM-HRMS data reveal a broad molecular weight distribution of sample components (up to 1100 m/z) and provide trendline isolation of feedstock components from those introduced “in process.” Chemical formulas were obtained from HRMS exact mass measurements (with typical mass error less than 5 ppm) and were consistent with structural carbohydrates and other lignocellulosic degradation products. Analyte assignments are supported via IM-MS collision-cross-section measurements and trendline analysis (e.g., all carbohydrate oligomers identified in a corn stover hydrolysate were found to fall within 6 % of an average trendline). These data represent the first report of collision cross sections for several negatively charged carbohydrates and other acidic species occurring natively in biomass hydrolysates.
ISSN 10440305
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-05-17
Publisher Institution The American Society for Mass Spectrometry
Publisher Place New York
e-ISSN 18791123
Journal Journal of The American Society for Mass Spectrometry
Volume Number 23
Issue Number 7
Page Count 10
Starting Page 1250
Ending Page 1259

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Source: SpringerLink