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Author Ramezani, Mohaddaseh ♦ Amoozegar, Mohammad Ali ♦ Ventosa, Antonio
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2014
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Halotolerant bacteria ♦ Oceanimonas ♦ Poly-β-hydroxybutyrate ♦ PHB pathway analysis ♦ Gavkhooni Wetland ♦ Iran ♦ Microbiology ♦ Microbial Genetics and Genomics ♦ Microbial Ecology ♦ Fungus Genetics ♦ Medical Microbiology ♦ Applied Microbiology
Abstract The screening of bacteria isolated from soil, water and sediment samples of the Gavkhooni Wetland in Iran led to the isolation of about 161 moderately halophilic and halotolerant bacteria which were able to accumulate intracellular lipid inclusions under conditions of nitrogen limitation. Primary studies showed that most of the isolates were able to produce these inclusions but that only a Gram-negative, rod-shaped halotolerant bacterium, designated as strain GK1(IBRC-M10197), showed high-capacity production in a wide range of culture conditions. Gas chromatography and Fourier transform infrared spectroscopy studies revealed that the inclusions were poly-β-hydroxybutyrate (PHB). Phenotypic characterization and phylogenetic analysis based on 16S rRNA gene sequence comparison showed that this strain is a member of the genus Oceanimonas. The genome sequence study of this bacterium revealed the presence of genes involved in the PHB synthesis pathway, which was supported by results of the transmission electron microscopy analysis. The isolate was capable of producing PHB under a wide range of physicochemical conditions, including pH 6–9, temperatures ranging from 20 to 40 °C and various salt concentrations and carbon/nitrogen sources. Under the optimum condition, cultivation in shake flasks (200 rpm) led to a PHB accumulation of about 75 wt% at 35 °C, pH 8 and 5 % (w/v) NaCl in a medium supplemented with 5 % sucrose as the carbon source and 0.5 % peptone as the nitrogen source.
ISSN 15904261
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-05-30
Publisher Place Berlin, Heidelberg
e-ISSN 18692044
Journal Annals of Microbiology
Volume Number 65
Issue Number 1
Page Count 10
Starting Page 517
Ending Page 526


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Source: SpringerLink