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Author Zhang, Zhuo ♦ Wang, Bin ♦ Hu, Shouping ♦ Zhang, Jiaoer ♦ He, Xijun ♦ Zheng, Shimin
Source SpringerLink
Content type Text
Publisher Springer India
File Format PDF
Copyright Year ©2015
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Aleutian disease virus ♦ Diagnosis ♦ Loop-mediated isothermal amplification ♦ Microbiology ♦ Life Sciences ♦ Biochemistry ♦ Protein Structure ♦ Cell Biology
Abstract In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and optimized for the detection of Aleutian disease virus (ADV) in minks. The amplification could be completed within 45 min under isothermal condition by employing a set of six ADV genome-specific primers. The amplification results could be visualized directly with the naked eye by using fluorescent dye. Comparative experiments showed that the LAMP assay is superior to conventional polymerase chain reaction for the detection of both experimental and field samples. Results of current study indicated that the LAMP assay is a rapid and reliable technique for routine diagnosis of ADV infection in minks.
ISSN 23473584
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-07-28
Publisher Place New Delhi
e-ISSN 23473517
Journal VirusDisease
Volume Number 26
Issue Number 3
Page Count 4
Starting Page 203
Ending Page 206

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Source: SpringerLink